What Is SNP Microarray SNP microarray, a specialized type of microarray technology, is geared towards the detection of Single Nucleotide Polymorphisms (SNPs), characterizing variations at a single base pair in the genome. Emerging in recent years, SNP microarray technology represents a high-throughput, large-scale genetic testing platform. The working principle largely entails the hybridization of fragmented single-stranded…
Cell-free DNA (cfDNA) has emerged as a pivotal biomarker in precision oncology, enabling non-invasive cancer diagnosis, personalized treatment, and real-time monitoring of tumour dynamics. cfDNA provides insights into tumor burden, genetic alterations, and epigenetic modifications, offering advantages over traditional tissue biopsies. Recent advancements in detection technologies have enhanced the sensitivity and specificity of cfDNA analysis, facilitating…
What is Microbial Sulfur Cycling? Sulfur, an indispensable nutrient for all life forms, serves as a vital building block for amino acids such as methionine and cysteine. Abundant sulfur reservoirs exist in nature, particularly in the marine ecosystem, where sulfate and sulfide are stored in copious amounts. The transformation of sulfur compounds with varying valence…
The use of next-generation amplicon sequencing technology has sparked debates within the microbiome research community due to its inherent limitations, primarily its short read length and the subsequent scarcity of information it provides. The taxonomic precision of marker genes is a pivotal factor directly influencing the reliability and credibility of microbiome function prediction. Therefore, a…
For cat lovers, the most tragic thing is probably the cat allergy. As soon as you get close to the cat, you sneeze, have a runny nose, or itchy eyes, skin rashes, and even asthma attacks. There are many people who are allergic to cats, with about 1 in 10 in the population. In some areas,…
Influenza A Virus (IAV) is one of the most important causes of respiratory infections and has caused tens of millions of deaths in several pandemic outbreaks throughout human history. In recent years, the recurrence of influenza epidemics in poultry and swine flocks has posed a serious threat to public health safety, thus, research on the…
What is Analytical Ultracentrifugation? The application of analytical ultracentrifugation (AUC), a formidable technique employed in the realm of biochemistry and polymer science, has paved the way for an in-depth analysis of the physical attributes of macromolecules in solution. By exploiting the centrifugal force, AUC successfully partitions molecules based on their sedimentation rates, a phenomenon that has…
What is BioID Technique? BioID (proximity-dependent biotin identification) is a proximity dependent labeling technology established by Roux et al in 2015 to study protein interactions. It can also be developed to study the interactions between proteins and RNA, and proteins and DNA. How BioID technology works? A recombinant expression vector of the target protein with biotin…
What is m6A Modification? Eukaryotic RNA contains over 160 modifications, primarily found on tRNAs and rRNAs. These modifications include m1A, m6A, m7G, m5C, and others. However, due to technological limitations, only a few types of modifications, such as “m6A (N6-methyladenosine)” and m5C, have been extensively studied, with m6A being the most researched. m6A refers to…
Introduction to Acetylation in RNA Histone acetylation has long been established as a mechanism for changing nucleosome structure. Direct links between transcription, histone acetylation, and the mechanisms by which histone acetylation is regulated have been formed in recent years. A substantial body of knowledge has accumulated regarding the mechanism(s) by which histone acetylation affects RNA…
