qPCR Analysis: qPCR Protocol and How a qPCR Machine Works
What is qPCR, and what steps are involved in developing qPCR protocol and analysis? The polymerase chain reaction (PCR)—a groundbreaking technique conceived by Kary Mullis, who received the Nobel Prize in Chemistry in 1992—transformed the landscape of molecular biology by facilitating rapid DNA amplification. Shortly thereafter, Russell Higuchi unveiled quantitative real-time PCR (qPCR), which employs…