What is Analytical Ultracentrifugation? The application of analytical ultracentrifugation (AUC), a formidable technique employed in the realm of biochemistry and polymer science, has paved the way for an in-depth analysis of the physical attributes of macromolecules in solution. By exploiting the centrifugal force, AUC successfully partitions molecules based on their sedimentation rates, a phenomenon that has…

What is BioID Technique? BioID (proximity-dependent biotin identification) is a proximity dependent labeling technology established by Roux et al in 2015 to study protein interactions. It can also be developed to study the interactions between proteins and RNA, and proteins and DNA. How BioID technology works? A recombinant expression vector of the target protein with biotin…

Protein-protein interactions, commonly known as PPIs, are of utmost importance in various biological processes, including but not limited to signal transduction, gene expression, and metabolic pathways. The complexity and sheer significance of PPIs require us to comprehend them in their entirety to unravel the mechanisms underlying these processes and generate innovative drugs for treating diseases….

Multimeric protein complexes are essential components of many cellular processes. These complexes consist of multiple proteins that work together to perform specific functions. To better understand these complexes, researchers use various techniques to identify and characterize the individual proteins that make up these complexes. One powerful tool for studying protein complexes is tandem affinity purification-mass spectrometry…